未経産ブタの摘出子宮内で培養したブタおよびヤギ精子の形態的変化

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  • Morphological changes of boar and goat spermatozoa incubated in the isolated uterus from a maturing gilt.
  • ミ ケイサン ブタ ノ テキシュツ シキュウナイ デ バイヨウシタ ブタ オヨ

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We reported previously that boar and goat spermatozoa incubated in the isolated uterus from a maturing gilt were able to penetrate into zona-free hamster eggs and that only acrosome reacted boar spermatozoa fused with such eggs. The present experiment was to investigate ultrastructural changes that occurred in boar and goat spermatozoa during incubation.<BR>Ejaculated spermatozoa from a Landrace boar and Saanen goat were washed twice, resuspended and incubated for 5h in a modified Krebs-Ringer bicarbonate (m-KRB) solution or in the isolated uterus from a maturing gilt. They were recovered from the uterus by flushing and the flushings were gently centrifuged at 50g for 5min to remove blood cells and epithelial cell debris. The super-natants containing the spermatozoa were centrifuged again at 500g for 10min. The sedimented spermatozoa were fixed in 2.5% cold glutaraldehyde for 1h, followed by fixation with 1% osmium tetroxide for 1h, and dehydrate in acetone. Ultrathin sections of the spermatozoa embedded in a mixture of Epon 812 and Epon 815 were stained with lead citrate and uranyl acetate, then examined with a JEM 100C electron microscope.<BR>Boar spermatozoa incubated in the m-KRB medium retained intact acrosomes. In contrast, boar and goat spermatozoa incubated in the isolated uterus from a maturing gilt exhibited various morphologi-cal changes, e.g., vacuolation of the acrosomal contents, appearance of microtuble like structures in the acrosomal cap region, and membrane vesiculation of the acrosomal cap region similar to the acrosome reaction. Such acrosome reactions were observed in 42.5% of boar and 20% of goat spermatozoa. The equatorial segment of acrosome reacted boar spermatozoa remained intact, but that of goat was almost lost. These results suggest that the uterus isolated from a maturing gilt has a capacity of inducing an acrosome reaction in spermatozoa of homologous (boar) or heterogous (goat) species.

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