インビトロにおけるウサギ胞状卵胞内卵母細胞の滑面小胞体の形態変化に及ぼすＬＨ添加の影響

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  • The effect of LH on morphological events of smooth-surfaced endoplasmic reticulum in rabbit follicular oocytes cultured in vitro
  • インビトロ ニ オケル ウサギ ホウジョウ ランポウナイ ランボ サイボウ ノ

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The effect of LH on structural characteristics of smooth-surfaced endoplasmic reticulum (SER) in oocytes liberated from well-developed antral follicles of rabbit ovaries was investigated in vitro. Large vesicular-typed SER, of which lumen contained flocculent material being moderate in electron density, were distributed uniformly throughout the cytoplasm of the oocytes before cultivation. The SER remained essentially unchanged when cultured in a medium with or without LH for either 12 or 24 hours. After 12 hour-culture including LH, small vesicular-typed SER, of which lumen contained electron-lucent material, appeared in the cortical ooplasm in addition to the above-mentioned SER. The newly-appeared SER, however, was not found in the ooplasm after 24 hour-culture. It will be concluded that the appearance of the small vesicular-typed SER depends on the presence of LH in the medium.

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