マウスと家との２細胞期はいより分離した単一割球の発達能および凍結生存性に関する研究

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  • Studies on the developmental ability and the viability after deep freezing of the blastomeres isolated from 2-cell stage embryos in mice and rabbits.
  • マウス ト カト ノ 2 サイボウキ ハイ ヨリ ブンリシタ タンイツ カッキ

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Developmental ability and viability after deep freezing of the blastomeres of mouse and rabbit embryos isolated at 2-cell stage were investigated by culturing these blastomeres in vitro. The blastomeres used were obtained by microsurgically dividing the 2-cell stage embryos along a inter-blastomeric groove with a fine glass needle. The zonae pellucidae were remained open or absent during the culture and the storage period.<BR>The successful dichotomies, which gave a pair of surviving blastomeres, were achieved in 81 out of 114 (71.7%) 2-cell embryos of mice and in 43 out of 62 (69.4%) 2-cell embryos of rabbits.;Forty-four out of 48 (91.7%) rabbit blastomeres developed into morphologically normal blasto-cysts, though they were significantly smaller in size than the respective control blastocysts derived from intact 2-cell embryos. In the mouse, 33 out of 87 (37.9%) blastomeres developed into 2-cell stage in culture.<BR>The mouse blastomeres survived after deep freezing at -80C. Ninety-one out of 100 (91.0%) blastomeres maintained normal morphology after freezing and thawing. Of these 91 blastomeres recovered in normal appearance, 73 (80.2%) developed in vitro into the 2-cell stage. In the rabbit, 28 out of 80 (35.0%) blastomeres were recovered in normal shape but only in one case a cleavage division into the 2-cell stage was observed after thawing.

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