培養ブタ卵胞卵における“核の形成”と分割

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  • 佐藤 英明
    京都大学農学部帯広畜産大学
  • 入谷 明
    京都大学農学部帯広畜産大学
  • 西川 義正
    京都大学農学部帯広畜産大学 Obihiro University of Agriculture and Veterinary Medicine

書誌事項

タイトル別名
  • "Formation of nucleus" and "cleavage" of pig follicular oocytes cultured in vitro
  • バイヨウ ブタ ランポウ ラン ニ オケル カク ノ ケイセイ ト ブンカツ

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抄録

Experiments were carried out to examine "formation of nucleus" and "cleavage" in pig follicular oocytes cultured in vitro. When oocytes with corona cells were cultured in modified Krebs Ringer bicarbonate solution, "formation of nucleus" was observed at 5760 hours, and at 27 hours in the oocytes without corona cells. The predominantly found numbers of "nucleus" in the oocytes were one and two. Extrusion of the second polar body was not observed, but the oocytes having two "polar bodies" were observed. "Cleavage" occurred at 72 hours of culture in modified Krebs Ringer bicarbonate solution. Fragmenting ova which closely resemble segmenting ova were observed, the predominantly found type of "cleaved" ova was 2 "cell", and 3 and 4 "cell" ova were also observed. When oocytes were cultured in Eagle MEM, "formation of nucleus" was observed at 69 hours of culture.

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