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- MORIOKA Hiroshi
- Labora-tory of Animal Reproduction, College of Agricul-ture, University of Osaka Prefecture
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- ICHIKAWA Shigetaka
- Labora-tory of Animal Reproduction, College of Agricul-ture, University of Osaka Prefecture
Bibliographic Information
- Other Title
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- ハムスターの体外培養卵巣からの排卵
- ハムスター ノ タイガイ バイヨウ ランソウ カラ ノ ハイラン
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Abstract
An improved culture method for the hamster ovary was developed to study the mechanism of ovulation. Hamsters used in this study spontaneously ovulated a mean number of 6.2 ova per ovary between 00:00 h and 02:00 h of the day of estrus. The ovaries were excised between 17:00 h of the day of proestrus and 01:00 h of the day of estrus, placed on a stainless steel wire table and cultivated at the surface of the culture medium of modified M-199 until 10:30 h of the following morning. Ova shed into the medium were counted. Among ten ovaries explanted at 17:00 h only one ovary ovulat-ed. The ovulation rate increased when the ovary was explanted at 18:00 h (47%) and 19:00 h (78%)and reached 100% at 21:00 h or later. When the ovaries were explanted at 21:00 h and 23:00 h of proestrus, the average number of shed ova was 4.7 and 4.6, respectively. These values are 75% of the value in in vivo ovulations. The ovaries explanted at 21:00 h of proestrus ovulated between 02:00 h and 06:00 h while the ovaries explanted at 00:00 h completed ovulation by 02:00 h.
Journal
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- The Japanese journal of animal reproduction
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The Japanese journal of animal reproduction 28 (4), 200-204, 1982
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
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Details 詳細情報について
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- CRID
- 1390282681310503040
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- NII Article ID
- 130004213997
- 40004157110
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- NII Book ID
- AN00041843
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- NDL BIB ID
- 2574078
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- ISSN
- 03859932
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed