牛・豚・犬・家兎・ラットおよびマウス精子における蛍光小体（Ｆ‐ｂｏｄｙ）の検出

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  • Detection of fluorescent body in spermatozoa of bull, boar, dog, rabbit, rat and mouse.
  • ウシ ブタ イヌ カト ラット オヨビ マウス セイシ ニ オケル ケイコウ

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An attempt was made to identify the fluorescent body (F-body), with Quina-crine mustard (Q-M) staining, in the spermatozoa from 7 mammalian species (human, bull, boar, dog, rabbit, rat and mouse). Washed sperm suspension in PBS was either stained with Q-M solution for 40 min or 24 hr, or treated with Protease and then stained with Q-M for 40 or 100 min. The final concentration of Q-M in sperm suspension was 0.025 mg/ml. The microscopic examination, using a reflected fluorescence microscope (B-exciter filter; 390490 nm, Optical suppression filter; ?? 510 nm), revealed that the same F-body as that in human sperms was present in all of these species. After 24 hr staining, F-body was found in 28.5, 28.4, 36.2, 3.0, 9.7 and 9.3% of sperms of bull, boar, rabbit, dog, rat and mouse, respectively. Enzyme treated specimens showed higher inci-dences (43.8, 44.0, 47.6 and 41.0% for bull, boar, rabbit and dog, respectively, and 20.4 and 20.7% for mouse and rat, and 32.5% for human) of the F-body than those in the specimens stained 24 hr without enzymatic digestion. These findings led to the conclusion that the F-body is present in common with spermatozoa of many mammalian species including man and gorila.<BR>The successful detection of F-bodies in the sperms from various mammalian species is attributed to; (1) the long-term submersion of specimens in the Q-M solution for staining and (2) the treatment of the membrane by protein digestion which yielded more favorable condition for staining in many animal species.

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