Sex-Reversed Somatic Cell Cloning in the Mouse
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- INOUE Kimiko
- BioResource Center, RIKEN Graduate School of Life and Environmental Sciences, University of Tsukuba
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- OGONUKI Narumi
- BioResource Center, RIKEN
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- MEKADA Kazuyuki
- BioResource Center, RIKEN
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- YOSHIKI Atsushi
- BioResource Center, RIKEN
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- SADO Takashi
- Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University
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- OGURA Atsuo
- BioResource Center, RIKEN Graduate School of Life and Environmental Sciences, University of Tsukuba The Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo
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Abstract
Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 55 (5), 566-569, 2009
The Society for Reproduction and Development
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Keywords
Details 詳細情報について
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- CRID
- 1390282681312368000
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- NII Article ID
- 10025616710
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 10412828
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed