卵細胞質注入法によるM期あるいはG1期に同期化した牛胎子繊維芽細胞由来再構築胚の核相の変化と初期発生
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- IDETA Atsushi
- ZEN-NOH Embryo Transfer Center
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- URAKAWA Manami
- ZEN-NOH Embryo Transfer Center
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- AOYAGI Yoshito
- ZEN-NOH Embryo Transfer Center
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- SAEKI Kazuhiro
- Department of Genetic Engineering, Kinki University
書誌事項
- タイトル別名
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- Early Morphological Nuclear Events and Developmental Capacity of Embryos Reconstructed with Fetal Fibroblasts at the M or G1 Phase after Intracytoplasmic Nuclear Injection in Cattle
この論文をさがす
抄録
We examined morphological nuclear events during the first cell cycle of bovine embryos reconstructed with somatic cells at the M and G1 phases (M-embryos and G1-embryos, respectively) by intracytoplasmic nuclear injection, and the subsequent development of these embryos in vitro and in vivo. Bovine fetal fibroblasts (BFFs) at the M or G1 phase were directly injected into enucleated oocytes, and activated immediately. Only half (48%) of the M-embryos extruded polar body-like cells (PBCs) at 6 h post injection (hpi). At 15 to 19 hpi, 54% of the M-embryos formed a single pronucleus-like nucleus. Nuclear envelope-breakdown, premature chromosome condensation and single nuclear clusters were observed in most of the G1-embryos (88%) within 30 min following the nuclear injection. At 15 to 19 hpi, single pronucleus-like nuclei were formed in most G1-embryos (83%). The potential of G1-embryos to develop to blastocysts was significantly higher than that of M-embryos (31% vs 16%). Three of five recipients following transfer of blastocysts derived from the G1-embryos became pregnant on Day 30, and one recipient delivered a calf. Our results indicate that almost a half of the M-embryos failed to extrude PBCs and that the G1-embryos developed to blastocysts at a higher rate than the M-embryos.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 51 (2), 187-194, 2005
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312425216
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- NII論文ID
- 220000098796
- 130000054345
- 10027324235
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 7305439
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- PubMed
- 15599113
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可