Hematoxylin Staining Reveals a Decrease in Nucleolar Diameter of Pig Oocytes Before Germinal Vesicle Breakdown
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- NOBATA Tadatoshi
- Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
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- KYOUGOKU Hirohisa
- Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
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- MIYANO Takashi
- Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
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During oocyte growth, the morphology of the nucleolus changes into a compact and homogenous structure. The compact nucleoli in full-grown oocytes are not stained by aceto-orcein staining or immunofluorescence staining. In this study, we developed a hematoxylin staining method for pig oocytes in whole-mount preparations to visualize the nucleoli. Nucleoli of growing and full-grown oocytes were stained blue with hematoxylin. Using this staining method, the changes in the oocyte nucleolus during maturation were examined. The nucleolar diameter gradually decreased in maturing oocytes (10.7 ± 0.1 μm to 9.0 ± 0.7 μm, P<0.05) before germinal vesicle breakdown (GVBD). The results suggest that the nucleolar volume of oocytes decreases before GVBD.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 59 (5), 500-505, 2013
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312450816
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- NII論文ID
- 130003361049
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC3sfgvFeqtQ%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 024943185
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- PubMed
- 23856597
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可