The In Vitro Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter
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- KAUFFOLD Johannes
- Large Animal Clinic for Theriogenology and Ambulatory Services, Faculty of Veterinary Medicine
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- AMER Hussein A. H.
- Large Animal Clinic for Theriogenology and Ambulatory Services, Faculty of Veterinary Medicine Department for Theriogenology, Zagazig University
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- BERGFELD Uwe
- Saxon State Institute of Agriculture
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- WEBER Wolfgang
- Department of Obstetrics and Gynecology, Phillip-Rosenthal-Strasse 55, University of Leipzig
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- SOBIRAJ Axel
- Large Animal Clinic for Theriogenology and Ambulatory Services, Faculty of Veterinary Medicine
書誌事項
- タイトル別名
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- The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter
- In Vitro Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter
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This study investigated the relationship between follicle size (FS) and developmental competence of calf oocytes. Cumulus-oocyte-complexes (COCs) from follicles >8 (L-COCs; n=19), 4-8 (M-COCs; n=54), and 2-3 mm (S-COCs; n=155) were recovered from non-stimulated 1-4 months old dairy calves post mortem and ex vivo (laparoscopy), and in parallel from slaughtered adult cows from follicles of identical size categories [> 8 (n=91); 4-8 (n=138); 2-3 mm (n=193)]. Morphologically intact COCs were subjected to in vitro maturation, fertilization, and embryo culture. Cleavage rate (CR; 46 h post-insemination=p.i.), rate of morulae/blastocysts (M/Bl; day 7 p.i.), and blastocysts (Bl; day 9 p.i.) were recorded. FS had no effect on the CR in calves. However, calf L-COCs yielded the highest rates of M/Bl and Bl compared with the two other size categories (P<0.05). In contrast, calf S- and M-COCs gave similar rates of M/Bl, whereas the proportion of Bl was lowest for S-COCs (P<0.05). This was almost identical to findings in cows, except that the CR was highest for L-COCs and M/Bl yields were lowest for S-COCs (P<0.05). There were no differences between calf and cows with regard to CR for the respective FS categories. L-COCs from calves and cows yielded similar rates of M/Bl and Bl, whereas calf S- and M-COCs yielded lower rates of Bl than S- and M-COCs from cows and a lower rate of M/Bl when S-and M-COCs were analyzed as one group (P<0.05). Whereas the CR was similar in calves and cows, calf COCs yielded lower rates of M/Bl and Bl (P<0.05). In conclusion, the results show that the developmental competence of calf oocytes is higher in those derived from follicles larger than 8 mm, and thus are almost equally as competent as cow oocytes derived from follicles of identical size. This suggests that calf oocytes acquire developmental competence within the large follicle, potentially due to a process similar to prematuration of the oocyte in the adult cow. It is proposed that procedures that facilitate prematuration, such as "coasting" following a preceding superstimulation, might increase the developmental competence of calf oocytes.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 51 (3), 325-332, 2005
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312525568
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- NII論文ID
- 10027324815
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 7345590
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- PubMed
- 16000866
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可