Transcription Factors GATA-4 and GATA-6 : Molecular Characterization, Expression Patterns and Possible Functions During Goose (Anser cygnoides) Follicle Development
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- YUAN Xin
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- XIA Lu
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- DONG Xia
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- HU Shenqiang
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- ZHANG Yingying
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- DING Fang
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- LIU Hehe
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- LI Liang
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
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- WANG Jiwen
- Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan 625014, P.R. China
書誌事項
- タイトル別名
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- Transcription Factors GATA-4 and GATA-6: Molecular Characterization, Expression Patterns and Possible Functions During Goose (<i>Anser cygnoides</i>) Follicle Development
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説明
The transcription factors GATA-4 and GATA-6, members of the GATA family, play an important role in ovarian cell proliferation, differentiation and apoptosis. In this study, the full-length coding sequences of goose GATA-4 and GATA-6 were cloned and characterized. GATA-4 and GATA-6 consist of 1236 and 1104 nucleotides encoding proteins with 411 and 367 amino acids, respectively. The deduced amino acid sequences of both proteins include two adjacent zinc finger domains with the distinctive form (CVNC-X17-CNAC)-X29-(CANC-X17-CNAC) and share 84.76% identity within this domain. In silico prediction together with matching of the high affinity RRXS(T)Y motif revealed that the GATA-4 protein might be phosphorylated predominantly at S233, but no phosphorylation site was found in the GATA-6 protein. Real-time quantitative PCR analysis showed that GATA-4 and GATA-6 mRNAs were co-expressed in goose follicles, moderately expressed in granulosa cells and weakly expressed in theca cells. The expression level of GATA-4 mRNA in healthy follicles was significantly higher than in atretic follicles or postovulatory follicles (P<0.01), and the expression level of GATA-6 mRNA in healthy follicles was significantly lower than in atretic follicles or postovulatory follicles (P<0.01). The expression level of GATA-4 mRNA in granulosa cells was downregulated during follicle development; the peak of expression occurred in the 8-10 mm follicles, and the lowest expression was in the F1 follicles. GATA-6 was upregulated and reached its peak expression in the F1 follicles. These results indicate that the molecular structural differences in goose GATA-4 and GATA-6 may be related to their different roles during follicle development.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (2), 83-91, 2014
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312664448
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- NII論文ID
- 130003390818
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2cvlsVSqtg%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 025409572
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- PubMed
- 24531706
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可