Resveratrol Improves the Mitochondrial Function and Fertilization Outcome of Bovine Oocytes
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- TAKEO Shun
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
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- SATO Daichi
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
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- KIMURA Koji
- National Institute of Livestock and Grassland Science, Tochigi 329-2793, Japan
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- MONJI Yasunori
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
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- KUWAYAMA Takehito
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
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- KAWAHARA-MIKI Ryoka
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
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- IWATA Hisataka
- Tokyo University of Agriculture, Kanagawa 243-0034, Japan
書誌事項
- 公開日
- 2014
- 資源種別
- journal article
- DOI
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- 10.1262/jrd.2013-102
- 公開者
- 公益社団法人 日本繁殖生物学会
この論文をさがす
説明
The aim of the present study was to address the effect of resveratrol-mediated upregulation of sirtuin 1 (SIRT1) during oocyte maturation on mitochondrial function, the developmental ability of oocytes and on mechanisms responsible for blockage of polyspermic fertilization. Oocytes collected from slaughterhouse-derived ovaries were cultured in TCM-199 medium supplemented with 10% FCS and 0 or 20 µM resveratrol (Res). We examined the effect of Res on SIRT1 expression in in vitro-matured oocytes (Exp 1); fertilization and developmental ability (Exp 2); mitochondrial DNA copy number (Mt number), ATP content and mitochondrial membrane potential in matured oocytes (Exp 3); and the time required for proteinase to dissolve the zona pellucida following in vitro fertilization (as a marker of zona pellucida hardening), as well as on the distribution of cortical granules before and after fertilization (Exp 4). In Exp 1, the 20 µM Res treatment upregulated protein expression of SIRT1 in oocytes. In Exp 2, Res treatment improved the ratio of normal fertilization and the total cell number of blastocysts. In Exp 3, Res treatment significantly increased the ATP content in matured oocytes. Additionally, Res increased the overall Mt number and mitochondrial membrane potential, but the effect was donor-dependent. In Exp 4, Res-induced zona hardening improved the distribution and exocytosis of cortical granules after in vitro fertilization. In conclusion, Res improved the quality of oocytes by improving mitochondrial quantity and quality. In addition, Res added to the maturation medium enhanced SIRT1 protein expression in oocytes and improved fertilization via reinforcement of the mechanisms responsible for blockage of polyspermic fertilization.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (2), 92-99, 2014
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312670592
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- NII論文ID
- 130003381865
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2czhvFamsw%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 025409574
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- PubMed
- 24390595
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
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- PubMed
- CiNii Articles
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- OpenAIRE
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- 使用不可
