TGF-β1 system in Leydig cells (part 2) TGF-β1 and progesterone, through Smad 1/5, are involved in the hyperplasia/hypertrophy of Leydig cells
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- GONZALEZ Candela R.
- Instituto de Biologíia y Medicina Experimental
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- GONZALEZ Betina
- Instituto de Biologíia y Medicina Experimental
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- RULLI Susana B.
- Instituto de Biologíia y Medicina Experimental
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- dos SANTOS Mara L.
- Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais
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- MATTOS JARDIM COSTA Guilherme
- Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais
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- FRANÇA Luiz R.
- Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais
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- CALANDRA Ricardo S.
- Instituto de Biologíia y Medicina Experimental
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- GONZALEZ-CALVAR Silvia I.
- Instituto de Biologíia y Medicina Experimental Facultad de Medicina, Universidad de Buenos Aires
書誌事項
- タイトル別名
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- TGF-.BETA.1 System in Leydig Cells. Part II: TGF-.BETA.1 and Progesterone, Through Smad1/5, are Involved in the Hyperplasia/hypertrophy of Leydig Cells
- TGF v 1 system in Leydig cells part 2 TGF v 1 and progesterone through Smad 1 5 are involved in the hyperplasia hypertrophy of Leydig cells
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抄録
Several reports indicate that transforming growth factor β1 (TGF-β1) participates in the regulation of cell cycle progression. In this work, we analyzed the in vitro effect of TGF-β1 on Leydig cell proliferation markers and the in vivo effect of this cytokine in Leydig cell hyperplasia/hypertrophy. The in vitro effect of TGF-β1 (1 ng/ml) plus progesterone (10-6 M) on purified Leydig cells from 3 week-old mice increased the immunocytochemically detected PCNA and stimulated the phosphorylation of Smad 1/5. Progesterone (10-6 M) in the presence or absence of TGF-β1 diminished the ratio Bax/Bcl-2. Morphometric testicular studies of mice treated with progesterone (s.c.) plus TGF-β1 (intratesticular), showed an increase in interstitial volume and a decrease in tubular volume. Furthermore, the cytoplasmic volume of Leydig cells showed an increment in this experimental group with a diminution in nuclear volume. Thus, it turned out that the administration of progesterone and TGF-β1 augmented the volume of Leydig cells. These results indicate a clear effect of TGF-β1 in the hypertrophy/hyperplasia of Leydig cells.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56 (4), 400-404, 2010
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312678912
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- NII論文ID
- 10026621587
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 10788747
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可