TGF-β1 system in Leydig cells (part 1) Effect of hCG and progesterone
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- GONZALEZ Candela R.
- Instituto de Biología y Medicina Experimental
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- GONZALEZ Betina
- Instituto de Biología y Medicina Experimental
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- RULLI Susana B.
- Instituto de Biología y Medicina Experimental
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- HUHTANIEMI Ilpo
- Department of Reproductive Biology, Imperial College of London
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- CALANDRA Ricardo S.
- Instituto de Biología y Medicina Experimental
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- GONZALEZ-CALVAR Silvia I.
- Instituto de Biología y Medicina Experimental Facultad de Medicina, Universidad de Buenos Aires
書誌事項
- タイトル別名
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- TGF-.BETA.1 System in Leydig Cells. Part I: Effect of hCG and Progesterone
- TGF v 1 system in Leydig cells part 1 Effect of hCG and progesterone
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抄録
Transforming growth factor beta 1 (TGF-β1) modulates male reproductive function. Genetically modified mice overexpressing α/β subunits of hCG (hCG+) show Leydig cell hyperplasia/hypertrophy at prepuberty that disappears as the mice approach adulthood. In this study we analyzed the gene expression of TGF-β1, its specific receptors, type II (TGF-βRII) and type I (activin receptor-like kinase 1 and 5: ALK1 and ALK5), and co-receptor endoglin (CD105) in purified Leydig cells from hCG+ and wild-type mice at 3 and 8 weeks of age and the occurrence of TGF-β1, ALK1 and ALK5 by immunohistochemistry. The expression of TGF-β1 was higher in hCG+ mice at both ages studied, and no changes were observed in TGF-βRII. ALK5 diminished with age in wild-type mice, whereas ALK1 decreased in hCG+ mice at 8 weeks of age. Endoglin expression showed a marked increase in 3-week-old hCG+ animals. In vitro incubation of Leydig cells from wild-type animals with hCG (10 IU/ml) increased TGF-β1 and ALK5 expression. Progesterone (10-6 M) induced endoglin expression. These studies provide novel evidence for differential gene and protein expression of ALK1 and ALK5 at different ages and endoglin expression and hormonal, in purified Leydig cells.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56 (4), 389-395, 2010
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312681728
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- NII論文ID
- 10026621527
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 10788716
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可