Usefulness of a Non-invasive Reporter System for Monitoring Reprogramming State in Pig Cells: Results of a Cell Fusion Experiment
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- OZAWA Akio
- Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University
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- AKASAKA Eri
- Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University
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- WATANABE Satoshi
- Animal Genome Research Unit, Division of Animal Science, National Institute of Agrobiological Sciences
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- YOSHIDA Mitsutoshi
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University
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- MIYOSHI Kazuchika
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University
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- SATO Masahiro
- Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University
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Dedifferentiation of differentiated cells such as fibroblasts into pluripotent stem cells, so-called iPS cells, was first reported by Yamanaka et al., who successfully employed retroviral gene delivery of four stem-cell-specific transcription factors (Oct-3/4, Klf4, Sox2 and c-myc). Despite the mouse system in which an Oct-3/4 or Nanog promoter-based reporter system has already been established, there is no useful system in pigs for reporting the reprogramming state of gene-engineered cells. In this study, we constructed a pOEIN plasmid carrying a ca. 5.4-kb mouse Oct-3/4 promoter linked to the EGFP cDNA and neomycin expression unit and produced a porcine embryonic cell line stably incorporating it in the genome. Cell fusion with mouse embryonal carcinoma cell line F9 resulted in generation of colonies with distinct EGFP-derived fluorescence around 14 days after fusion. RT-PCR using these colonies also confirmed expression of endogenous porcine pluripotency-specific Oct-3/4, Sox2 and Stat3 mRNA. These findings suggest that mouse-derived components are sufficient to induce dedifferentiation of differentiated pig cells and also that reprogramming proceeds gradually. The present non-invasive reporter system will be useful to better define the reprogramming mechanism and/or to identify novel reprogramming molecules in the pig.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56 (4), 363-369, 2010
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681312685952
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- NII論文ID
- 10026621377
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 10788615
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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