Genetic Influences in Mouse Spermatogonial Stem Cell Self-Renewal
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- KANATSU-SHINOHARA Mito
- Department of Molecular Genetics, Graduate School of Medicine, Kyoto University
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- OGONUKI Narumi
- The Institute for Physical and Chemical Research (RIKEN), Bioresource Center
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- MIKI Hiromi
- The Institute for Physical and Chemical Research (RIKEN), Bioresource Center
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- INOUE Kimiko
- The Institute for Physical and Chemical Research (RIKEN), Bioresource Center
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- MORIMOTO Hiroko
- Department of Molecular Genetics, Graduate School of Medicine, Kyoto University
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- TAKASHIMA Seiji
- Department of Molecular Genetics, Graduate School of Medicine, Kyoto University
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- OGURA Atsuo
- The Institute for Physical and Chemical Research (RIKEN), Bioresource Center
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- SHINOHARA Takashi
- Department of Molecular Genetics, Graduate School of Medicine, Kyoto University Japan Science and Technology Agency, CREST
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Abstract
Spermatogonial stem cells (SSCs) are slowly dividing cells that undergo self-renewal division to support spermatogenesis. Although the effects of genetic background in stem cell self-renewal have been well studied in hematopoietic stem cells, little is known about its effect on stem cells in other self-renewing tissues, including SSCs. To examine whether genetic factors are involved in regulation of SSC self-renewal, we first studied spermatogenesis in different inbred mouse strains (C57BL/6, DBA/2, AKR, BALB/C and C3H) after chemical damage caused by busulfan. Spermatogenesis in the DBA/2 and AKR strains was relatively resistant to busulfan treatment, whereas spermatogenesis was diminished in C57BL/6 mice and nearly ablated in C3H and BALB/C mice. Serial germ cell transplantation experiments provided functional evidence that SSCs with the DBA/2 background expanded more rapidly than those with the B6 background. Finally, we also employed the Germline Stem (GS) cell culture technique to examine the self-renewal activity in vitro. Although genetic manipulation of GS cells has been limited to those from the DBA/2 background, we produced transgenic offspring of the C3H background by electroporation of GS cells with a plasmid vector. Our results underscore the importance of genetic factors in SSC self-renewal. Furthermore, application of genetic modification techniques to GS cells with non-DBA/2 backgrounds extends the potential of a SSC-based approach in male germline modification.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56 (1), 145-153, 2010
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681312975360
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- NII Article ID
- 10026323942
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 10581819
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed