Production of Trophoblastic Vesicles Derived From Day 7 and 8 Blastocysts of In Vitro Origin and the Effect of Intrauterine Transfer on the Interestrous Intervals in Japanese Black Heifers
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- NAGAI Kaya
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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- SATA Ryuichi
- Tochigi Prefectural Daily Farming Experiment Station
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- TAKAHASHI Hitomi
- National Institute of Livestock and Grassland Science
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- OKANO Akira
- National Institute of Livestock and Grassland Science
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- KAWASHIMA Chiho
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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- MIYAMOTO Akio
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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- GESHI Masaya
- National Institute of Livestock and Grassland Science
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This study was undertaken to produce trophoblastic vesicles (TVs) by using blastocysts of in vitro origin and to estimate the effect on the interestrous interval after transfer of 4 TVs into the uteri of heifers on Day 7. Morphological examination under a stereoscopic microscope revealed that the total formation rate of TVs prepared from IVP expanded blastocysts was 80.5% and that there was no difference in the formation rates of TVs derived from blastocysts between Day 7 (83.5%) and Day 8 (78.5%). After intrauterine transfer of TVs, observation of the corpus luteum (CL) by transrectal ultrasonography together with measurement of the plasma progesterone concentration confirmed that 2 of 4 recipients (50%) had a longer interestrous interval, 33.5 and 35.0 days, while the other 2 recipients had normal cycles, 20.0 and 24.5 days. In the control group transferred D-PBS, all 4 heifers had a normal cycles, 24.0-24.5 days. Consequently, the average number of days after intrauterine transfer of TVs compared with the 2 consecutive cycles just before the treatment was longer than in the controls (6.1 ± 2.4 days vs. -0.8 ± 1.1 days, P<0.05). These results indicate that preparation of TVs from blastocysts of in vitro origin is a useable method and that TVs from blastocysts may have the capacity to maintain CL function after intrauterine transfer.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 55 (4), 454-459, 2009
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313070848
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- NII論文ID
- 10025194593
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BD1MrptFSnsA%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 10329102
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- PubMed
- 19420837
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可