SRD Outstanding Research Award 2011 : Isolation of Ovarian Components Essential for Growth and Development of Mammalian Oocytes In Vitro
-
- HIRAO Yuji
- Livestock and Forage Research Division, Tohoku Agricultural Research Center (TARC), National Agriculture and Food Research Organization (NARO), Morioka 020-0198, Japan
書誌事項
- タイトル別名
-
- Isolation of Ovarian Components Essential for Growth and Development of Mammalian Oocytes <i>In Vitro</i>
- Isolation of ovarian components essential for growth and development of mammalian oocytes in vitro
この論文をさがす
抄録
Mammalian ovaries contain a large number of oocytes, most of which degenerate either before or at various stages of growth. Dynamic and precise regulation in the ovary involves many factors, each with a unique role. Identifying the single most important factor is impossible; however, it may be possible to identify factors essential for oocyte growth. It is evident that oocytes can grow into competent ova in vitro; however, how faithfully the follicle should mimic the in vivo conditions remains unclear. In the culture system discussed in this review, bovine and mouse oocyte-granulosa cell complexes, at approximately the late mid-growth stage, spread on a substratum without the involvement of theca cells. The structural simplicity of this system is advantageous because it reduces the basic conditions essential for regulation of oocyte growth. Apart from biological factors, high concentrations of polyvinylpyrrolidone (molecular weight: 360000) improved oocyte growth. Among ovarian factors, androstenedione was used to compensate for the absence of theca cells, and it promoted both follicular growth and acquisition of oocyte meiotic competence. Most oocytes cultured in a group were viable after long-term culture, suggesting that unlike ovarian events, there was no exhaustive follicle selection. Collectively, oocytes and their associated granulosa cells can establish independent units capable of supporting oocyte growth in appropriately modified culture media.
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 58 (2), 167-174, 2012
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681313394816
-
- NII論文ID
- 10030753405
-
- NII書誌ID
- AA10936678
-
- COI
- 1:STN:280:DC%2BC38jltlKktg%3D%3D
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 023601974
-
- PubMed
- 22738899
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可