Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos

DOI Web Site Web Site PubMed 1 Citations 28 References
  • HUAN Yan Jun
    Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China College of Life Science, Northeast Agricultural University, Haerbin 150030, China
  • ZHU Jiang
    College of Life Science, Northeast Agricultural University, Haerbin 150030, China College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
  • WANG Hong Mei
    Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China
  • WU Zhan Feng
    Shouguang City Hospital of Chinese Medicine, Weifang 262700, China
  • ZHANG Ji Guang
    Shouguang City Hospital of Chinese Medicine, Weifang 262700, China
  • XIE Bing Teng
    College of Life Science, Northeast Agricultural University, Haerbin 150030, China
  • LI Jing Yu
    College of Life Science, Northeast Agricultural University, Haerbin 150030, China
  • KONG Qing Ran
    College of Life Science, Northeast Agricultural University, Haerbin 150030, China
  • LIU Zhong Hua
    College of Life Science, Northeast Agricultural University, Haerbin 150030, China
  • HE Hong Bin
    Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China

Search this article

Description

Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2’-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.

Journal

Citations (1)*help

See more

References(28)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top