Production Efficiency and Telomere Length of the Cloned Pigs Following Serial Somatic Cell Nuclear Transfer
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- KUROME Mayuko
- Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilians-University Munich
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- HISATOMI Hisashi
- Laboratory of Cellular and Molecular Biochemistry, Department of Materials and Life Science, Seikei Universty
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- MATSUMOTO Shirou
- Department of Pediatrics, Graduate School of Medical Science, Kumamoto University
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- TOMII Ryo
- Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University
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- UENO Satoshi
- Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University
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- HIRUMA Katsumi
- Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University
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- SAITO Hitoshi
- Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University
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- NAKAMURA Kimitoshi
- Department of Pediatrics, Graduate School of Medical Science, Kumamoto University
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- OKUMURA Kenji
- Department of Pediatrics, Graduate School of Medical Science, Kumamoto University
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- MATSUMOTO Mitsuhito
- National Agricultural Research Center for Kyushu Okinawa Region
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- KAJI Yuji
- National Agricultural Research Center for Kyushu Okinawa Region
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- ENDO Fumio
- Department of Pediatrics, Graduate School of Medical Science, Kumamoto University
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- NAGASHIMA Hiroshi
- Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University
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Abstract
The aim of the present study was to examine the production efficiency of cloned pigs by serial somatic cell nuclear transfer (SCNT) and to ascertain any changes in the telomere lengths of multiple generations of pigs. Using fetal fibroblasts as the starting nuclear donor cells, porcine salivary gland progenitor cells were collected from the resultant first-generation cloned pigs to successively produce second- and third-generation clones, with no significant differences in production efficiency, which ranged from 1.4% (2/140) to 3.3% (13/391) among the 3 generations. The average telomere lengths (terminal restriction fragment values) for the first, second and third generation clones were 16.3, 18.1 and 20.5 kb, respectively, and were comparable to those in age-matched controls. These findings suggest that third-generation cloned pigs can be produced by serial somatic cell cloning without compromising production efficiency and that the telomere lengths of cloned pigs from the first to third generations are normal.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 54 (4), 254-258, 2008
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681313514752
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- NII Article ID
- 10024289316
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 9621639
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed