SRD young investigator award: Factors affecting fertilization and embryonic development during intracytoplasmic sperm injection in pigs

  • NAKAI Michiko
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • KASHIWAZAKI Naomi
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
  • ITO Junya
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
  • MAEDOMARI Naoki
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
  • OZAWA Manabu
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • SHINO Masao
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University
  • NOGUCHI Junko
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • KANEKO Hiroyuki
    Division of Animal Sciences, National Institute of Agrobiological Sciences
  • KIKUCHI Kazuhiro
    Division of Animal Sciences, National Institute of Agrobiological Sciences

書誌事項

タイトル別名
  • Factors Affecting Fertilization and Embryonic Development During Intracytoplasmic Sperm Injection in Pigs

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抄録

In intracytoplasmic sperm injection (ICSI) technique, a sperm was injected into ooplasm directly using a glass pipette. The fertilization physiology in ICSI is considered quite different from that of the natural fertilization. The different mechanisms for fertilization may be the causes of various results in ICSI. In this paper, we focus on the state of sperm membranes, nuclear or DNA integrity during ICSI procedure and discuss the influence of these factors on fertilization and embryonic development. We also introduce some examples in application of ICSI for new technologies in pigs.<br>

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