ウシ黄体における黄体形成ホルモン(LH)のホスホリパーゼCを介したプロジェステロン分泌促進機構に関する研究
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- NISHIMURA Ryo
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- SHIBAYA Masami
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- SKARZYNSKI Dariusz J.
- Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Science
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- OKUDA Kiyoshi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
書誌事項
- タイトル別名
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- Progesterone Stimulation by LH Involves the Phospholipase-C Pathway in Bovine Luteal Cells
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抄録
Luteinizing hormone (LH)-stimulated steroidogenesis in luteal cells is known to be mediated through the activation of cyclic AMP (cAMP)-dependent protein kinase, and to be also modulated by calcium-dependent mechanisms. In the present study, we tested the hypothesis that LH stimulates progesterone (P4) production in bovine luteal cells through activation of phospholipase (PL) C by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to a PLC inhibitor (U-73122; 10 μM) with or without LH (10 ng/ml) for 4 h. U-73122 blocked LH-stimulated P4 production without affecting cAMP accumulation. Moreover, exposure of luteal cells to PLC increased P4 production in a dose-dependent manner. These results support the hypothesis that the luteotropic action of LH in bovine luteal cells is mediated not only by activation of adenylate cyclase but also by activation of PLC.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 50 (2), 257-261, 2004
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313564672
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- NII論文ID
- 10027322285
- 220000092724
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 6917283
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- PubMed
- 15118253
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- 本文言語コード
- en
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- データソース種別
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- IRDB
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- 使用不可