Progesterone Stimulation by LH Involves the Phospholipase-C Pathway in Bovine Luteal Cells
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- NISHIMURA Ryo
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- SHIBAYA Masami
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- SKARZYNSKI Dariusz J.
- Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Science
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- OKUDA Kiyoshi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
Bibliographic Information
- Other Title
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- ウシ黄体における黄体形成ホルモン(LH)のホスホリパーゼCを介したプロジェステロン分泌促進機構に関する研究
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Abstract
Luteinizing hormone (LH)-stimulated steroidogenesis in luteal cells is known to be mediated through the activation of cyclic AMP (cAMP)-dependent protein kinase, and to be also modulated by calcium-dependent mechanisms. In the present study, we tested the hypothesis that LH stimulates progesterone (P4) production in bovine luteal cells through activation of phospholipase (PL) C by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to a PLC inhibitor (U-73122; 10 μM) with or without LH (10 ng/ml) for 4 h. U-73122 blocked LH-stimulated P4 production without affecting cAMP accumulation. Moreover, exposure of luteal cells to PLC increased P4 production in a dose-dependent manner. These results support the hypothesis that the luteotropic action of LH in bovine luteal cells is mediated not only by activation of adenylate cyclase but also by activation of PLC.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 50 (2), 257-261, 2004
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681313564672
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- NII Article ID
- 10027322285
- 220000092724
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 6917283
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- PubMed
- 15118253
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed