Transgenic Expression of Testis-Specific Poly(A) Polymerase TRAP in Wild-Type and TPAP-Deficient Mice
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- ZHUANG Tiangang
- Institute of Applied Biochemistry, University of Tsukuba
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- KASHIWABARA Shin-ichi
- Institute of Applied Biochemistry, University of Tsukuba
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- NOGUCHI Junko
- Germ Cell Conservation Laboratory, National Institute of Agrobiological Sciences
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- BABA Tadashi
- Institute of Applied Biochemistry, University of Tsukuba
Bibliographic Information
- Other Title
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- 精巣特異的ポリAポリメラーゼTPAPトランスジーンの野生型とTPAP欠損マウスでの発現
- Transgenic Expression of Testis-Specific Poly(A) Polymerase TPAP in Wild-Type and TPAP-Deficient Mice
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Description
We have previously identified a testis-specific poly(A) polymerase, TPAP (PAPβ), involved in poly(A) tail extension of specific mRNAs in the cytoplasm of round spermatids. Targeted disruption of the mouse TPAP gene resulted in the arrest of spermiogenesis due to reduced expression of haploid-specific genes required for morphogenesis of germ cells. To further elucidate the role(s) of TPAP in spermatogenesis, transgenic mice expressing an exogenous TPAP transgene on wild-type and TPAP-deficient backgrounds were generated and characterized. The transgenic mice overexpressing TPAP exhibited normal spermatogenesis and fertility. The sizes of some transcription factor mRNAs as the substrates of TPAP were also unaffected. Transgenic expression of the TPAP gene in the TPAP-deficient mice complemented both the incomplete elongation of the poly(A) tails of specific transcription factor mRNAs, and reduced expression of haploid-specific genes, resulting in the resumption of normal spermiogenesis. These data conclusively show that spermatogenesis requires the cytoplasmic elongation of the mRNA poly(A) tails catalyzed by TPAP, and imply the presence of a regulatory mechanism(s) defining the extent of the cytoplasmic mRNA polyadenylation.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 50 (2), 207-213, 2004
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681313570816
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- NII Article ID
- 10027322123
- 220000092719
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 6917221
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- PubMed
- 15118248
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed