Detection of Zearalenone and Its Metabolites in Naturally Contaminated Porcine Follicular Fluid by Using Liquid Chromatography-Tandem Mass Spectrometry
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- SAMBUU Rentsenkhand
- United Graduate School of Veterinary Science, Yamaguchi University Research Institute of Animal Husbandry
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- TAKAGI Mitsuhiro
- Faculty of Agriculture, Kagoshima University
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- SHIGA Satoshi
- Faculty of Agriculture, Kagoshima University
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- UNO Seiichi
- Faculty of Fisheries, Kagoshima University
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- KOKUSHI Emiko
- Faculty of Fisheries, Kagoshima University
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- NAMULA Zhao
- United Graduate School of Veterinary Science, Yamaguchi University
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- OTOI Takeshige
- United Graduate School of Veterinary Science, Yamaguchi University
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- MIYAMOTO Akio
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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- DEGUCHI Eisaburo
- Faculty of Agriculture, Kagoshima University
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- FINK-GREMMELS Johanna
- Faculty of Veterinary Medicine, Utrecht University
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抄録
Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. We aimed to simultaneously detect ZEN and its metabolites á-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in porcine follicular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and α-ZOL, but not β-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter ≥ 6 mm) within 10 ovaries. Furthermore, ZEN and α-ZOL were detected in samples pretreated with β-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 57 (2), 303-306, 2011
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313625088
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- NII論文ID
- 10028275704
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 11058553
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可