Intratesticular Expression of mRNAs of Both Interferon γ and Tumor Necrosis Factor α is Significantly Increased in Experimental Autoimmune Orchitis in Mice
-
- TERAYAMA Hayato
- Department of Anatomy, Tokyo Medical University
-
- NAITO Munekazu
- Department of Anatomy, Tokyo Medical University
-
- QU Ning
- Department of Anatomy, Tokyo Medical University
-
- HIRAI Shuichi
- Department of Anatomy, Tokyo Medical University
-
- KITAOKA Miyuki
- Department of Anatomy, Tokyo Medical University
-
- OGAWA Yuki
- Department of Anatomy, Tokyo Medical University
-
- ITOH Masahiro
- Department of Anatomy, Tokyo Medical University
書誌事項
- タイトル別名
-
- Intratesticular Expression of mRNAs of Both Interferon γ and Tumor Necrosis Factor α is Significantly Increased in Experimental Autoimmune Orchitis in Mice
- Intratesticular expression of mRNA s of both interferon g and tumor necrosis factor a is significantly increased in experimental autoimmune orchitis in mice
この論文をさがす
抄録
Experimental autoimmune orchitis (EAO) is one of the models of immunological male infertility. Murine EAO is CD4+T cell-dependent and classically induced by immunization with a testicular homogenate and adjuvants. We previously established that immunization with viable syngeneic testicular germ cells (TGC) can also induce murine EAO with no use of any adjuvant. Analyses of this EAO model have already revealed that cultured spleen cells of immunized mice secreted interferon (IFN)-γ and that treatment of the immunized mice with anti-IFN-γ monoclonal antibodies significantly suppressed the EAO. It is known that both IFN-γ and tumor necrosis factor (TNF)-α are representative cytokines of Th1 cells and exhibit local toxicity toward the seminiferous epithelium in vivo. However, changes in these two cytokines in EAO-affected testes have not yet been investigated. Therefore, in the present study, we investigated the expression of intratesticular IFN-γ and TNF- α mRNAs in TGC-induced EAO using real-time RT-PCR. The results demonstrated that the intratesticular mRNAs for both IFN-γ and TNF-α significantly increased, while other cytokines such as IL-1α, IL-1β, IL-6 and TGF-β did not show dramatic changes in the immunized mice. These results suggest that secretion of significant amounts of IFN-γ and TNF-α in situ contributes to the spermatogenic disturbance in EAO.<br>
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 57 (2), 296-302, 2011
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681313626880
-
- NII論文ID
- 10028275659
-
- NII書誌ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 11058521
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可