DNA Methylation Status of Bovine Blastocyst Embryos Obtained from Various Procedures
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- SAWAI Ken
- Faculty of Agriculture, Iwate University
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- TAKAHASHI Masashi
- Department of Animal and Grassland Research, National Agricultural Center for Kyushu Okinawa Region
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- FUJII Takashi
- Faculty of Agriculture, Iwate University
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- MORIYASU Satoru
- Hokkaido Animal Research Center
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- HIRAYAMA Hiroki
- Hokkaido Animal Research Center
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- MINAMIHASHI Akira
- Hokkaido Animal Research Center Hokkaido Konsen Agricultural Experiment Station
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- HASHIZUME Tsutomu
- Faculty of Agriculture, Iwate University
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- ONOE Sadao
- Hokkaido Animal Research Center
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抄録
DNA methylation is an important factor for the regulation of gene expression in early embryos. It is well known that the satellite I sequence is more heavily methylated in bovine somatic cell nuclear transfer (NT-SC) embryos than in embryos derived from in vitro fertilization (IVF). However, the methylation status of bovine embryos obtained by other procedures is not well known. To clarify DNA methylation levels of bovine embryos obtained from various procedures, we examined satellite I sequences in bovine blastocyst (BC) embryos derived from NT-SC, NT using embryonic blastomeres (NT-EM), in vivo (Vivo), IVF and parthenogenetic treatment (PA). Furthermore, in order to evaluate the efficacy of DNA demethylation by the NT procedure, we determined the DNA methylation levels in bovine embryos in which NT was recapitulated (Re-NT). Although the DNA methylation levels in the NT-SC embryos were higher than those in the other embryos, the NT-EM embryos exhibited lower DNA methylation levels. The satellite I sequence in the NT-SC embryos was more demethylated than that in the donor cells. Although the DNA methylation level in the individual NT-SC embryos showed variation, the full-term developmental efficacy of these embryos were not different. These findings suggest that the methylation level of the satellite I sequence at the BC stage is not related to the abnormalities of bovine embryos produced by NT-SC. There was no difference in methylation levels between Re-NT and NT-SC embryos. Our results indicated that the DNA methylation status differed among embryos produced by various methods and that at least some of the demethylation of the donor cell genome occurred in the recipient cytoplast after NT-SC, but the demethylation ability of the NT procedure was noted in the first NT but not in the second NT.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 57 (2), 236-241, 2011
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313649664
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- NII論文ID
- 10028275322
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 11058290
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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