Inhibition of cathepsin B activity reduces apoptosis by preventing cytochrome <i>c</i> release from mitochondria in porcine parthenotes
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- KIM Seon-Hyang
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- ZHAO Ming-Hui
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- LIANG Shuang
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- CUI Xiang-Shun
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
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- KIM Nam-Hyung
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Republic of Korea Brain Korea 21 Center for Bio-Resource Development, Chungbuk 361-763, Republic of Korea
Bibliographic Information
- Other Title
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- Inhibition of cathepsin B activity reduces apoptosis by preventing cytochrome c release from mitochondria in porcine parthenotes
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Abstract
Cathepsin B, a lysosomal cysteine protease of the papain family, has recently been implicated in the quality and developmental competence of bovine preimplantation embryos. In this study, to determine whether inhibition of cathepsin B activity can improve porcine oocyte maturation and early embryo developmental competence, we supplemented in vitro maturation or embryo culture media with E-64, a cathepsin B inhibitor. Cathepsin B activity was high in poor quality germinal vesicle stage oocytes, but no differences in mRNA expression or protein localization were observed between good and poor quality oocytes, which were categorized based on morphology. Following treatment with 1 μM E-64, cathepsin B activity sharply decreased in 4-cell and blastocyst stage embryos. E-64 had no effect on cell number but significantly (P < 0.05) increased blastocyst formation and decreased the number of apoptotic cells in blastocysts. It also significantly (P < 0.05) enhanced mitochondrial membrane potential in blastocysts, reducing the release of cytochrome c and resulting in decreased expression of Caspase-3 and Caspase-9. In conclusion, inhibition of cathepsin B activity in porcine parthenotes using 1 μM E-64 resulted in attenuation of apoptosis via a reduction in the release of cytochrome c from mitochondria.
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 61 (4), 261-268, 2015
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681313753856
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- NII Article ID
- 130005094645
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 026727907
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- PubMed
- 25903788
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed