Mice Cloned by Nuclear Transfer from Somatic and ntES Cells Derived from the Same Individuals
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- WAKAYAMA Sayaka
- Center for Developmental Biology, RIKEN Kobe Department of Life Science, Graduate School of Science and Technology, Kobe University
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- MIZUTANI Eiji
- Center for Developmental Biology, RIKEN Kobe Graduate School of Agricultural Science, Tohoku University
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- KISHIGAMI Satoshi
- Center for Developmental Biology, RIKEN Kobe
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- THUAN Nguyen Van
- Center for Developmental Biology, RIKEN Kobe
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- OHTA Hiroshi
- Center for Developmental Biology, RIKEN Kobe
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- HIKICHI Takafusa
- Center for Developmental Biology, RIKEN Kobe
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- BUI Hong Thuy
- Center for Developmental Biology, RIKEN Kobe
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- MIYAKE Masashi
- Department of Life Science, Graduate School of Science and Technology, Kobe University
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- WAKAYAMA Teruhiko
- Center for Developmental Biology, RIKEN Kobe
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説明
The current success rate of cloned mice from adult somatic cell nuclei is very low, whereas it is relatively high for cloned mice from ES cell nuclei. In this experiment, we examined whether the success rate of cloning from somatic cells could be improved via nuclear transfer embryonic stem cells (ntES cells) established from somatic cell nuclei. We obtained 11 cloned mice and 68 ntES cell lines from the somatic cell nuclei of 7 mice, and cloned 41 mice were cloned from the ntES cell nuclei. Unexpectedly, the overall success rate of cloning from ntES cell nuclei in this series was no better than when using somatic cell nuclei. Interestingly, full-term cloned mice were produced only via ntES cells from two individuals, but not by direct nuclear transfer from the somatic cells, and vice versa. Ultimately, we were able to obtain clone mice from 6 out of 7 individuals using either somatic cells or ntES cells. Thus, although ntES cells as donor nuclei do not absolutely assure a better success rate for mouse cloning than somatic cells, to preserve and clone valuable individuals, we recommend that ntES cell lines be established. These can then be used as an unlimited source of donor nuclei for nuclear transfer, and thus complement conventional somatic cell nuclear transfer cloning approaches.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 51 (6), 765-772, 2005
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313902720
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- NII論文ID
- 130000054680
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 7755402
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- PubMed
- 16227673
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- 本文言語コード
- en
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- データソース種別
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- NDLサーチ
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