A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of <i>in vitro</i> produced porcine embryos
-
- BARTOLAC Louise Katherine
- Faculty of Veterinary Science, The University of Sydney, NSW 2570, Australia Westmead Fertility Centre, Westmead Hospital, Westmead, NSW 2145, Australia
-
- LOWE Jenna Louise
- Faculty of Veterinary Science, The University of Sydney, NSW 2570, Australia
-
- KOUSTAS George
- Westmead Fertility Centre, Westmead Hospital, Westmead, NSW 2145, Australia
-
- SJÖBLOM Cecilia
- Westmead Fertility Centre, Westmead Hospital, Westmead, NSW 2145, Australia
-
- GRUPEN Christopher Gerald
- Faculty of Veterinary Science, The University of Sydney, NSW 2570, Australia
Bibliographic Information
- Other Title
-
- A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
Search this article
Abstract
The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either superfine open-pulled straws (SOPS) or the CryoLoopTM system, or vitrified in a closed device using either the CryoTipTM or Cryo BioTM’s high security vitrification system (HSV). The post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%; CryoLoopTM: 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices (CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting (76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture (16.0%) prior to vitrification were significantly less likely to survive vitrification than the control (non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to vitrification did not improve survival, which is inconsistent with the findings of studies in other species. This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the collapsing procedures.
Journal
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 61 (6), 525-531, 2015
The Society for Reproduction and Development
- Tweet
Details 詳細情報について
-
- CRID
- 1390282681313932288
-
- NII Article ID
- 130005113569
-
- NII Book ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL BIB ID
- 026989252
-
- PubMed
- 26211782
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed