Production of Chimaeric Mice Derived from Embryonic Stem Cells (F1/1) Which Have Neomycin Phosphotransferase (neo) Gene Introduced by Electroporation

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  • ネオマイシンリン酸転移酵素(neo)遺伝子を導入した胚性幹細胞(F1/1)のキメラ形成能の検討
  • ネオマイシンリンサン テンイ コウソ neo イデンシ オ ドウニュウシタ ハ

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Non-129 embryonic stem cells (F1/1) were transformed with the neomycin phosphot-ranscerase (neo) gene by electroporation and used as a vehicle for transgenesis. There was no difference between square wave and exponential decay in transformation efficiency of F1/1 cells by selection for G418 resistance. Southern blot hybridization analysis has confirmed that 4 out of 6 neomycin resistant ES cell lines have neo gene sequences in their genomic DNA. The karyotype of these cells appeared stable, as they showed euploidy after transformation. Overt coat color chimaeras were obtained from all 4 neo resistant ES cell lines tested.

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