Aging of Recipient Oocytes Reduces the Development of Cloned Embryos Receiving Cumulus Cells
-
- LIU Guohui
- Laboratory of Animal Reproduction, College of Agriculture, Kinki University
-
- KATO Yoko
- Laboratory of Animal Reproduction, College of Agriculture, Kinki University
-
- TSUNODA Yukio
- Laboratory of Animal Reproduction, College of Agriculture, Kinki University
この論文をさがす
抄録
Parthenogenetic activation is an important factor in successful production of cloned mammals. Because it has been reported that aged oocytes are more sensitive to parthenogenetic activation than young oocytes, the present study examined the effects of oocyte aging on the in vitro and in vivo developmental potential of nuclear-transferred (NT) mouse oocytes receiving cumulus cells. The potentials of young NT oocytes (14 h after human chorionic gonadotrophin [hCG] injection) to develop into blastocysts was, however, significantly higher than that of aged oocytes (20 h after hCG injection; 16% vs 6%). When the nuclei of NT oocytes at the 2-cell stage were fused with enucleated fertilized 2-cell embryos, the potentials of the serial NT embryos to develop into blastocysts were no different for both young and aged oocytes (74% vs 74%). Live young, however, were obtained only after transfer of serial NT blastocysts developed from young NT oocytes (2%). In contrast to a report using embryonic nuclei as the nuclear donors, the results of the present study indicate that young oocytes are superior to aged oocytes as a source of recipient cytoplasm for mouse somatic cell cloning.<br>
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 53 (4), 785-790, 2007
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681314173184
-
- NII論文ID
- 10020003770
-
- NII書誌ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 8882784
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可