Vitrification of Immature Bovine Oocytes by the Microdrop Method
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- KIM Dong-Hoon
- Animal Biotechnology Division, National Institute of Animal Science
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- PARK Hyo-Suk
- Animal Biotechnology Division, National Institute of Animal Science Department of Animal Science and Biotechnology, Chonnam National University
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- KIM Se-Woong
- Animal Biotechnology Division, National Institute of Animal Science
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- HWANG In-Sun
- Animal Biotechnology Division, National Institute of Animal Science
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- YANG Byoung-Chul
- Animal Biotechnology Division, National Institute of Animal Science
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- IM Gi-Sun
- Animal Biotechnology Division, National Institute of Animal Science
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- CHUNG Hak-Jae
- Animal Biotechnology Division, National Institute of Animal Science
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- SEONG Hwan-Woo
- Animal Biotechnology Division, National Institute of Animal Science
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- MOON Seung-Joo
- Department of Animal Science and Biotechnology, Chonnam National University
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- YANG Boh-Suk
- Animal Biotechnology Division, National Institute of Animal Science
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Abstract
This study was conducted to determine the optimal vitrification conditions for immature bovine oocytes using the microdrop method. In experiment 1, the optimal pre-equilibration period for microdrop vitrification was examined. The maturation rate of vitrified oocytes with a 3 min first pre-equilibration period (41.1%) was higher than that of vitrified oocytes with a 0 min first pre-equilibration period (21.4%), and the values of those with a 1 (33.9%) or 5 min (27.4%) first pre-equilibration period were intermediate. The value for a 1 min second pre-equilibration period (44.4%) was significantly higher (P<0.05) than those for a 0.5 (28.6%) and 2 min (21.4%) second pre-equilibration period. In experiment 2, the distribution of microtubules in matured oocytes was investigated. There was no difference among the first pre-equilibration times in terms of the rates of normal spindles in vitrified oocytes. However, this value was significantly higher (P<0.05) in the 1 min group (52.8%) compared with the 0.5 (16.7%) and 2 min groups (12.3%). In experiment 3, we investigated the developmental capacity of immature bovine oocytes vitrified under optimal pre-equilibration conditions (3 min and 1 min for the first and second pre-equilibrations, respectively). Although the total fertilization rates were significantly lower (P<0.05) in the vitrified oocytes (65.6%) compared with the control oocytes (92.4%), there was no difference in the rate of normal fertilization (2PN) between the vitrified (78.6%) and control (82.0%) oocytes. Cleavage and blastocyst rates were significantly lower (P<0.05) in vitrified oocytes (55.7 and 2.3%) than in control oocytes (84.4 and 34.7%). Thus, these results indicated that immature bovine oocytes can survive after microdrop vitrification and subsequently can be cultured to mature oocytes capable of undergoing fertilization in vitro and developing into blastocysts.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 53 (4), 843-851, 2007
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681314180480
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- NII Article ID
- 10020003983
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 8882919
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed