In Vitro Development of Equine Oocytes from Preserved Ovaries after Intracytoplasmic Sperm Injection
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- MATSUKAWA Kazutsugu
- Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
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- AKAGI Satoshi
- Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
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- ADACHI Noritaka
- Ibaraki Prefectural Livestock Research Center
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- SATO Fumio
- Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association
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- HASEGAWA Telhisa
- Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association
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- TAKAHASHI Seiya
- Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
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抄録
In this study, we evaluated the meiotic competence of equine oocytes from ovaries preserved for one day. We also investigated fertilization, cleavage rate, developmental competence and freezability of equine embryos after intracytoplasmic sperm injection (ICSI). After collection from ovaries, the oocytes were classified into two groups comprised of those having compact cumulus layers (Cp) or those having expanded cumulus layers (Ex). Oocytes with a first polar body were subjected to fertilization by ICSI using frozen-thawed stallion spermatozoa and were then cultured in CR1aa medium. The rates of metaphase II-stage oocytes, normal fertilization and cleavage were not significantly different between the two oocyte categories (38.5, 70.0 and 48.7% for CP and 43.5, 60.0 and 58.8% for Ex, respectively). However, the blastocyst development rate of Ex was significantly (P<0.05) higher than that of Cp (25.5 vs. 7.7%). Three Cp-derived and 12 Ex-derived early blastocysts were cryopreserved using the slow cooling protocol, and all of them developed to hatching blastocysts after thawing. These results suggest that equine oocytes fertilized by ICSI can develop to the preimplantation stage in culture conditions similar to those used in the bovine. Furthermore, the Ex oocytes had higher developmental competence than the Cp oocytes, and the in vitro-produced blastocysts had high viability after freezing and thawing.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 53 (4), 877-885, 2007
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314263296
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- NII論文ID
- 10020004104
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 8882985
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可