Estrogen Regulates the Serum Level of Phosphorylated Prolactin in Mice
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- HORIGUCHI Kotaro
- Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
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- FUKUTA Shinya
- Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
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- YOSHIDA Makoto
- Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
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- KOSUGI Tatsuya
- Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
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- NAITO Jun-ichiro
- Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
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- ISHIDA Michiyo
- Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
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- HARIGAYA Toshio
- Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
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Phosphorylated prolactin (PPRL) is considered to be the most quantitatively important post-translationally modified form of prolactin (PRL) in rodents. We recently detected two different types of PPRL in the mouse pituitary gland; one was phosphorylated at serine and the other was phosphorylated at serine/threonine. Furthermore, we showed that there are obvious differences in the ratios between PPRLs and non-phosphorylated PRL in the pituitary gland based on age and sex and that estrogen influences PRL phosphorylation at serine in female mice. In the present study, we examined whether estradiol (E2) increases serine PPRL in the male pituitary gland in the same manner as in the female pituitary gland and examined whether PPRL is released into serum. We first determined the relative amounts of intrapituitary PPRLs in male mice under different pharmacological conditions that increased PRL secretion. The results indicated that treatment with E2 increases serine PPRL. We then performed two-dimensional electrophoresis and immunoblotting analysis after immunoprecipitation with anti-mouse PRL antibody using male and female sera under different pharmacological conditions that increased PRL secretion. The results of this experiment indicated that there were PRLs phosphorylated at serine and serine/threonine in the female serum but not in the male serum. The levels of PPRLs in sera were greatly increased with the E2 treatment for both male and female sera. Furthermore, we examined the effect of E2 on PPRL synthesis in cultured male pituitary glands. In this experiment, we observed increased serine PPRL synthesis and stronger immunohistochemical staining of PRL cells with E2 treatment. These findings suggested that serine PPRL synthesis and secretion were influenced by estrogen.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 53 (4), 915-922, 2007
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314265856
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- NII論文ID
- 10020004269
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 8883069
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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