Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes After Parthenogenetic Activation and Somatic Cell Nuclear Transplantation
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- KERE Michel
- Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC
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- SIRIBOON Chawalit
- Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC
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- LO Neng-Wen
- Department of Animal Science and Biotechnology, Tunghai University, Taichung 407, Taiwan, ROC
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- NGUYEN Ngoc Tan
- Agricultural Biotechnology Center, Institute of Agricultural Science for Southern Vietnam, Ho Chi Minh City, Vietnam
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- JU Jyh-Cherng
- Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan, ROC Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC
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In this study, a dose-response assessment was performed to understand the relation between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte maturation and the in vitro development of parthenotes (PA) and handmade cloned (HMC) embryos. Various concentrations (0, 25, 50 and 100 μg/ml) of vitamin C supplemented in in vitro maturation (IVM) and culture (IVC) media were tested. None of these vitamin C additions affected nuclear maturation of oocytes, yet supplementation at 50 μg/ml led to significantly increased intracellular glutathione (GSH) levels and reduced reactive oxygen species (ROS). When cultured in IVM- and/or IVC-supplemented media, the group supplemented with 50 μg/ml of vitamin C showed improved cleavage rates, blastocyst rates and total cell numbers per blastocyst (P<0.05) compared with other groups (control, 25 μg/ml and 100 μg/ml). In contrast, supplementation with 50 μg/ml vitamin C decreased (P<0.05) the apoptosis index as compared with the groups supplemented with 100 μg/ml. In addition, even with a lower blastocyst rate to start with (37.6 vs. 50.3%, P<0.05), supplementation of HMC embryos with vitamin C ameliorated their blastocyst quality to the extent of PA embryos as indicated by their total cell numbers (61.2 vs. 59.1). Taken together, an optimized concentration of vitamin C supplementation in the medium not only improves blastocyst rates and total cell numbers but also reduces apoptotic indices, whereas overdosages compromise various aspects of the development of parthenotes and cloned porcine embryos.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 59 (1), 78-84, 2013
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314297088
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- NII論文ID
- 10031156815
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC3s7js1akuw%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 024263522
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- PubMed
- 23154385
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可