Downregulation of Histone Methyltransferase Genes SUV39H1 and SUV39H2 Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs

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  • DANG-NGUYEN Thanh Quang
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8577, Japan Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • HARAGUCHI Seiki
    Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • FURUSAWA Tadashi
    Developmental Biology Department, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
  • SOMFAI Tamas
    Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • KANEDA Masahiro
    Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • WATANABE Shinya
    Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • AKAGI Satoshi
    Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • KIKUCHI Kazuhiro
    Department of Animal Science, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
  • TAJIMA Atsushi
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8577, Japan
  • NAGAI Takashi
    Department of Animal Breeding and Reproduction, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan

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タイトル別名
  • Downregulation of Histone Methyltransferase Genes <i>SUV39H1</i> and <i>SUV39H2</i> Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs

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Telomere is a nucleoprotein structure at the ends of chromosomes that helps to protect the ends of chromosomes from being fused with other chromosomes. Knockout of histone methyltransferases Suv39h1 and Suv39h2 increases the telomere length in murine cells, whereas downregulation of SUV39H1 and SUV39H2 genes decreases the telomere length in human cells, suggesting that telomere biology is different among mammalian species. However, epigenetic regulation of the telomere has not been studied in mammals other than the human and mouse. In the present study, the effect of knockdown of SUV39H1 and SUV39H2 genes on telomere length was examined in porcine embryonic stem-like cells (pESLCs) and porcine embryonic fibroblasts (PEFs). The telomeres in SUV39H1 and SUV39H2 knockdown (SUV39KD) pESLCs (37.1 ± 0.9 kb) were longer (P<0.05) compared with those of the control (33.0 ± 0.7 kb). Similarly, SUV39KD PEFs had longer telomeres (22.1 ± 0.4 kb; P<0.05) compared with the control (17.8 ± 1.1 kb). Telomerase activities were not different between SUV39KD pESLCs (10.4 ± 1.7) and the control (10.1 ± 1.7) or between SUV39KD PEFs (1.0 ± 0.3) and the control (1.0 ± 0.4), suggesting that telomerase activities did not contribute to the telomere elongation in SUV39KD pESLCs and SUV39KD PEFs. Relative levels of trimethylation of histone H3 lysine 9 and expressions of DNMT1, DNMT3A and DNMT3B were decreased in SUV39KD cells, suggesting that telomere lengthening in SUV39KD pESLCs and SUV39KD PEFs might be not only related to the loss of histone modification marks but also linked to the decrease in DNA methyltransferase in pigs.

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