Expression of Endometrial Immune-related Genes Possibly Functioning During Early Pregnancy in the Mare
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- TACHIBANA Yurika
- Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Ibaraki 319-0206, Japan Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- NAKANO Yasuko
- Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- NAGAOKA Kentaro
- Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan
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- KIKUCHI Masato
- Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- NAMBO Yasuo
- Hidaka Training Research Center, Japan Racing Association, Hokkaido 057-0171, Japan
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- HANEDA Shingo
- Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
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- MATSUI Motozumi
- Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
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- MIYAKE Yo-ichi
- Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
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- IMAKAWA Kazuhiko
- Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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抄録
Despite enormous efforts, biochemical and molecular mechanisms associated with equine reproduction, particularly processes of pregnancy establishment, have not been well characterized. Previously, PCR-selected suppression subtraction hybridization analysis was executed to identify unique molecules functioning in the equine endometrium during periods of pregnancy establishment, and granzyme B (GZMB) cDNA was found in the pregnant endometrial cDNA library. Because GZMB is produced from natural killer (NK) cells, endometrial expression of GZMB and immune-related transcripts were characterized in this study. The level of GZMB mRNA is higher in the pregnant endometrium than in non-pregnant ones. This expression was also confirmed through Western blot and immunohistochemical analyses. IL-2 mRNA declined as pregnancy progressed, while IL-15, IFNG and TGFB1 transcripts increased on day 19 and/or 25. Analyses of IL-4 and IL-12 mRNAs demonstrated the increase in these transcripts as pregnancy progressed. Increase in CCR5 and CCR4 mRNAs indicated that both Th1 and Th2 cells coexisted in the day 25 pregnant endometrium. Taken together, the endometrial expression of immune-related transcripts suggests that immunological responses are present even before the trophectoderm actually attaches to the uterine epithelial cells.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 59 (1), 85-91, 2013
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314300160
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- NII論文ID
- 10031156816
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC3s7itVemtw%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 024263549
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- PubMed
- 23138119
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可