One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
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- FUJII Wataru
- Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- ONUMA Asuka
- Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- SUGIURA Koji
- Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- NAITO Kunihiko
- Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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Abstract
Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in all-alleles. F0 mice showed knockout phenotypes or no protein expression of target genes simultaneously, and these mutations were normally inherited in the next generation.
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (4), 324-327, 2014
The Society for Reproduction and Development
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Keywords
Details 詳細情報について
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- CRID
- 1390282681314394752
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- NII Article ID
- 130004137824
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- NII Book ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2M%2Fgs12nug%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 025629570
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- PubMed
- 25110137
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed