Sericin Accelerates the Production of Hyaluronan and Decreases the Incidence of Polyspermy Fertilization in Bovine Oocytes During In Vitro Maturation

DOI Web Site Web Site PubMed 被引用文献2件 参考文献55件
  • HOSOE Misa
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
  • YOSHIDA Nao
    Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan
  • HASHIYADA Yutaka
    National Livestock Breeding Center, Nishigo, Fukushima 961-8511, Japan
  • TERAMOTO Hidetoshi
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
  • TAKAHASHI Toru
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan Present: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan
  • NIIMURA Sueo
    Faculty of Agriculture, Niigata University, Niigata 950-2181, Japan

書誌事項

タイトル別名
  • Sericin Accelerates the Production of Hyaluronan and Decreases the Incidence of Polyspermy Fertilization in Bovine Oocytes During <i>In Vitro</i> Maturation

この論文をさがす

抄録

Fetal bovine serum (FBS) has been widely used as a supplement in the maturation medium of bovine oocytes in vitro. However, serum contains many undefined factors and is potentially infectious to humans and animals. As a serum replacement, we evaluated the feasibility of using the silk protein, sericin, derived from the cocoons of silkworm. To examine the rates of oocyte maturation and fertilization, cumulus-oocyte complexes were cultured in TCM-199 supplemented with 0.01%, 0.05%, 0.1% or 0.15% sericin or 5% FBS. The sizes of the perivitelline space that might relate to polyspermy, the expressions of Has2 and CD44 mRNA, the amount of hyaluronan (hyaluronic acid: HA) contained in the oocytes and the rates of blastocyst formation following insemination were then compared between the oocytes cultured with 0.05% sericin and 5% FBS, because the polyspermy rates in oocytes cultured with 0.05% sericin were significantly lower than in those cultured with 5% FBS. After in vitro maturation (IVM), the mean size of the perivitelline space was significantly greater in oocytes cultured with sericin than in those cultured with FBS, although the rates of nuclear maturation, fertilization and blastocyst formation of oocytes under both IVM conditions were not significantly different. The expression of HAS2 and CD44 mRNA and the amount of HA in the denuded oocytes cultured with 0.05% sericin were significantly greater than in those cultured with FBS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM in bovine oocytes.

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (55)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ