Effects of downregulating GLIS1 transcript on preimplantation development and gene expression of bovine embryos
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- TAKAHASHI Kazuki
- Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan
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- SAKURAI Nobuyuki
- The United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan
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- EMURA Natsuko
- Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan
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- HASHIZUME Tsutomu
- Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan The United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan
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- SAWAI Ken
- Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan The United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan
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Abstract
Krüppel-like protein Gli-similar 1 (GLIS1) is known as a direct reprogramming factor for the generation of induced pluripotent stem cells. The objective of this study was to investigate the role of GLIS1 in the preimplantation development of bovine embryos. GLIS1 transcripts in in vitro-matured oocytes and 1-cell to 4-cell stage embryos were detected, but they were either absent or at trace levels at the 8-cell to blastocyst stages. We attempted GLIS1 downregulation of bovine early embryos by RNA interference and evaluated developmental competency and gene transcripts, which are involved in zygotic gene activation (ZGA) in GLIS1-downregulated embryos. Injection of specific siRNA resulted in a distinct decrease in GLIS1 transcript in bovine embryos at the 4-cell stage. Although the bovine embryos injected with GLIS1-siRNA could develop to the 16-cell stage, these embryos had difficulty in developing beyond the 32-cell stage. Gene transcripts of PDHA1 and HSPA8, which are transcribed after ZGA, showed lower level in GLIS1 downregulated embryos. It is possible that GLIS1-downregulated embryos fail to initiate ZGA. Our results indicated that GLIS1 is an important factor for the preimplantation development of bovine embryos.
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 61 (5), 369-374, 2015
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390282681314415872
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- NII Article ID
- 130005104112
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 026812961
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- PubMed
- 26074126
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed