JSAR Outstanding Presentation Award 2005: Manipulation of Fish Germ Cell: Visualization, Cryopreservation and Transplantation
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- OKUTSU Tomoyuki
- Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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- YANO Ayaka
- Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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- NAGASAWA Kazue
- Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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- SHIKINA Shinya
- Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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- KOBAYASHI Terumasa
- Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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- TAKEUCHI Yutaka
- Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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- YOSHIZAKI Goro
- Department of Marine Biosciences, Tokyo University of Marine Science and Technology SORST, Japan Science and Technology Agency
書誌事項
- タイトル別名
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- Manipulation of Fish Germ Cell: Visualization, Cryopreservation and Transplantation
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説明
Germ-cell transplantation has many applications in biology and animal husbandry, including investigating the complex processes of germ-cell development and differentiation, producing transgenic animals by genetically modifying germline cells, and creating broodstock systems in which a target species can be produced from a surrogate parent. The germ-cell transplantation technique was initially established in chickens using primordial germ cells (PGCs), and was subsequently extended to mice using spermatogonial stem cells. Recently, we developed the first germ-cell transplantation system in lower vertebrates using fish PGCs and spermatogonia. During mammalian germ-cell transplantation, donor spermatogonial stem cells are introduced into the seminiferous tubules of the recipient testes. By contrast, in the fish germ-cell transplantation system, donor cells are microinjected into the peritoneal cavities of newly hatched embryos; this allows the donor germ cells to migrate towards, and subsequently colonize, the recipient genital ridges. The recipient embryos have immature immune systems, so the donor germ cells can survive and even differentiate into mature gametes in their allogeneic gonads, ultimately leading to the production of normal offspring. In addition, implanted spermatogonia can successfully differentiate into sperm and eggs, respectively, in male and female recipients. The results of transplantation studies in fish are improving our understanding of the development of germ-cell systems during vertebrate evolution.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 52 (6), 685-693, 2006
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314978816
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- NII論文ID
- 10020350577
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 8596585
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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