ラット培養脊髄後根神経節ニューロンにおけるノルアドレナリンのブラジキニン応答への影響―ホールセルパッチクランプ法による電気生理学的解析―

DOI Web Site
  • 前田 亮
    東京医科歯科大学大学院医歯学総合研究科口腔機能再構築学系専攻口腔機能再建学講座疼痛制御学分野 東京医科歯科大学難治疾患研究所先端分子医学研究部門自律生理分野

書誌事項

タイトル別名
  • Modulation of Bradykinin Responses by Noradrenaline in Cultured Neonatal Rat Dorsal Root Ganglion Neurons-Electorophysiological Analysis by Whole-Cell-Patch Clamp Method-
  • ラット バイヨウ セキズイ コウコン シンケイセツ ニューロン ニ オケル ノルアドレナリン ノ ブラジキニン オウトウ エノ エイキョウ ホールセル パッチ クランプホウ ニ ヨル デンキ セイリガクテキ カイセキ
  • —Electorophysiological Analysis by Whole-Cell-Patch Clamp Method—
  • ―ホールセルパッチクランプ法による電気生理学的解析―

この論文をさがす

抄録

It is widely accepted that the sympathetic nerve activity might participate in some mechanisms underlying neuropathic pain which sometimes develops after nerve injuries.<BR>Many chemical substances have been proposed to induce pain sensation. Bradykinin (BK) is the most potent in exciting nociceptors and in sensitizing them to thermal and mechanical stimuli. We investigated modulatory effects of sympathetic agents on BK actions in primary afferent dorsal root ganglion (DRG) cells. The present electrophysiological experiments by using the patch clamp technique were conducted to examine the effects of noradrenaline (NA) on BK responses and to determine the sites and mechanism of interaction between them.<BR>1. Small (diameter, 20-35μum) DRG neurons responded to BK with inducing inward currents (BK-responses), but no BK responses could be evoked in DRG neurons larger than 35μm.<BR>2 . NA at 10μM augmented the BK responses in amplitude compared to the control response.<BR>3 . Clonidine at 1μM augmented the BK responses in amplitude, but isoproterenol at 1μM failed to augment the BK responses.<BR>4. After pretreatment with yohimbine, the BK responses were not changed by NA.<BR>It is concluded that NA-induced augmentation of the BK actions occurred by activating a 2-adrenergic receptor. Furthermore, this experimental system is a useful model in order to study the cellular mechanisms of neuropathic pain.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ