Variability of Argyrophilic Nucleolar Organizer Regions in Osteoblastic Cells

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  • 骨芽細胞様細胞 MC3T3-E1 における AgNORs の変化

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The changes in the number of AgNORs per nucleus in normal cells are obscure. In the present study, we examined the variability of AgNORs in osteoblastic MC3T3-E1 cells. We designed to enumerate the number of AgNORs during cell differentiation. The cells were grown for up to 20 days in the presence of fetal bovine serum. At the days 3, 6, 9, 13 and 20 after plating, the cells were stained by AgNOR method and also used for measurement of ALP activity. The number of AgNORs per nucleus increased from 6.9±2.15 (at the day 3) to 11.7±2.79 (at the day 6), decreased to 7.9±2.25 (at the day 9) and then leveled to 7.7±2.19 (at the day 20). The ALP activity was maximal at the day 9 after plating. In osteoblastic cells, the number of AgNORs was maximized in the earlier matrix maturation stage, not in the proliferating stage and was variable during cell differentiation.

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