Effects of TGF-.BETA. and TGF-.BETA.-neutralizing Antibody on Normal Skin Fibroblasts and Scar-derived Fibroblasts.
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- YAMAUCHI TOSHIHIKO
- Department of Plastic and Reconstruction Surgery, Kurume University School of Medicine
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説明
Transforming growth factor-beta (TGF-β) is known as an important cytokine for scar formation in wound healing. The purpose of the present study was to investigate the effects of TGF-β and its neutralizing antibody on normal skin fibroblasts and scar-derived fibroblasts in culture. Endogenous TGF-β levels were similar in all fibroblasts. Cell proliferation increased when TGF-β1 or β2 was added to the cultures, and the increase was higher and started at a lower level in the scar-derived fibroblasts (p<0.05). The increase of fibroblasts was suppressed by the addition of TGF-β-neutralizing antibody to the cultures, and the suppression rate was higher in the scar-derived cells (p<0.05). Percentages of the cells in the growth phases of cell cycle decreased in the normal skin fibroblasts (p<0.05) when TGF-β-neutralizing antibody was added. Our findings showed that scar-derived fibroblasts and normal skin fibroblasts have a different sensitivity to TGF-β. Further study is needed on the effect of the neutralizing antibody to the cell counts and cell cycle of scarderived fibroblasts.
収録刊行物
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- Ｔｈｅ Ｋｕｒｕｍｅ Ｍｅｄｉｃａｌ Ｊｏｕｒｎａｌ
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Ｔｈｅ Ｋｕｒｕｍｅ Ｍｅｄｉｃａｌ Ｊｏｕｒｎａｌ 49 (4), 171-176, 2002
久留米大学医学部 The Kurume Medical Journal 編集部
