Induction of Proembryogenic Masses and Formation of Adventitious Buds from Mature Embryos of an Endangered Japanese Conifer, Picea koyamae

DOI Web Site 36 References
  • Nakagawa Remi
    Faculty of Agriculture, Tokyo University of Agriculture and Technology Tsukuba Research Institute, Sumitomo Forestry Co., LTD.
  • Funada Ryo
    Faculty of Agriculture, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • 絶滅危惧種ヤツガタケトウヒの成熟種子胚からの不定胚形成細胞および不定芽の誘導
  • ゼツメツ キグシュ ヤツガタケトウヒ ノ セイジュク シュシハイ カラ ノ フテイハイケイセイ サイボウ オヨビ フテイ ガ ノ ユウドウ

Search this article

Description

Picea koyamae is an endangered Japanese conifer and only one thousand individuals are identified. Tissue culture is one of the techniques useful to propagate endangered species effectively. We examined the effect of 2, 4-dichlorophenoxy acetic acid (2, 4-D) and benzylaminopurine (BAP) on organogenesis from mature embryos of P.koyamae. Proembryogenic masses (PEMs) like cells were induced on Lepoivre (LP) medium containing 3μM 2, 4-D and caulogenic callus were induced on LP medium containing 1μM 2, 4-D and 3μM BAP. Furthermore, adventitious buds were developed from caulogenic callus under light condition.

Journal

  • Mokuzai Gakkaishi

    Mokuzai Gakkaishi 56 (5), 333-338, 2010

    The Japan Wood Research Society

References(36)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top