大腸菌に対する色素の光力学作用のアクシヨン・スペクトルについて

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  • Studies on the Action Spectra for the Photodynamic Action of Some Kinds of Dyes on <i>Escherichia Coli</i>
  • ダイチョウキン ニ タイスル シキソ ノ コウリョクガク サヨウ ノ アクション スペクトル ニ ツイテ

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The photodynamic action of dyes on Escherichia coli was studied applying more than ten sorts of them known as photodynamic active or inactive and unknown.<br>The relation between the absorption spectra of dyes and the action spectra for the photodynamic action was examined.<br>The absorption spectra of the dyes were measured spectrophotometrically and the action spectra for the photosensitve dyes on E. coli were measured exposing the bacterial suspension of the dye solution to a series of monochromatic radiations at certain wave lengths applying interference filters, with a certain equivalent dosis.<br>The experimental results were followings.<br>1) Comparing the action spectra for the photodynamic action with the absorption spectra, three types of the action were classified.<br>The first type: The action spectrum agrees well with the absorption spectrum, namely the region of the maximum action corresponds to the region of the maximum absorption.<br>The second type: The phase of the action spectrum shifts to the region of the longer wave length, however the general tendency of the action spectrum and the absorption spectrum in similar.<br>The third type: The photodynamic action becomes linearly stronger with increase of the wavelength.<br>2) No common structure which characterizes the photodynamic substrate was found, namely even if the chemical structure resembles each other, it is determined that the one is active and the other is inactive.<br>3) Between the intensity of the fluorescence of dye and the photodynamic activity at a certain wavelength, no particular relation was found.<br>4) However, the structure which possesses a halogene radical at the ortho positon of quinone nucleus shows the photodynamic activity.

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