条虫免疫に関する研究

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  • Immunological studies on tapeworm
  • ジョウチュウ メンエキ ニ カンスル ケンキュウ 2
  • II) Hemagglutination test with tannic acid and Sparganum mansoni antigen treated red cells
  • II)マンソン孤虫抗原感作タンニン酸処理赤血球凝集反応

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Hemagglutination tests as described by Boyden were conducted for the detection of antibodies against Sparganum mansoni antigen.<br>Human blood cells were sensitized with tannic acid and coated with the anigen prepared from dried Sparganum mansoni worm by sonic disintegration.<br>Antisera were obtained from the rabbit immunized by intravenous injection of the antigen.<br>The following results were botained.<br>1. It was shown that antibodies in the immune serum were successfully titrated by the hemagglutination test.<br>2. The optimum concentration of tannic acid for the treatment of red cells was 1:50, 000 dilution. Red cells treated with 1:20, 000 dilution of tannic acid showed often non-specific agglutination. The reactions were negative, when red cells treated with 1:100, 000 dilution of tannic acid were used.<br>3. It was observed that 1:3, 000 dilution of the dried antigen was the adequate concentration to sensitize tannic acid cells, and the highest dilution of the antiserum for positive reaction was 1:1, 600.

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