Studies on the discriminating method of dead from live eggs of <i>Ascaris</i> applying fluorescence microscopy

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  • 螢光顕微鏡法を応用した回虫卵生死判別法の検討
  • ケイコウ ケンビキョウホウ オ オウヨウ シタ カイチュウ ランセイシ ハンベツホウ ノ ケントウ

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Abstract

Ascaris eggs (removed the albuminous coats) in a water solution of acridine orange were observed under the fluorescence microscope. In case of live eggs, the shells fluoresced in pale green or greenish yellow, and the contents of the eggs were not visible. However, in case of dead eggs, the contents fluoresced remarkably in green, greenish yellow, yellow, orange or red. The contents of uncompleted and unfertilized eggs had the same fluorescence as the dead eggs.<br>The authors applied this phenomenon to the discrimination of dead from live eggs of Ascaris, and resulted in success. This fluorescence method could be used during any stage of development of the eggs.<br>Furthermore, the cause of difference in the fluorescent images between live and dead eggs was studied. In live egg, the shell did not permit the permeation of fluorochrome, but in dead egg, the degeneration of the egg-cell brought out autofluorescence and due to the degeneration of the inner layer of shell the fluorochrome permeated into the egg and brought out secondary fluorescence of the contents.<br>By the employment of the fluorescence method with the culture method together, the most rapid and exact discrimination may be possible.

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