Adenosine 3′, 5′-monophosphate (cyclic-AMP) in a persistently infected Vero cells line with measles virus

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  • Arima Yasuo
    Department of Microbiology and Immunology, Nippon Medical School

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  • 麻しんウイルス持続感染Ｖｅｒｏ細胞におけるａｄｅｎｏｓｉｎｅ３′，５′‐ｍｏｎｏｐｈｏｓｐｈａｔｅについて
  • マシン ウイルス ジゾク カンセン Vero サイボウ ニ オケル adeno

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Adenosine 3′, 5′-monophosphate (cyclic-AMP) level in Vero-S cell line, which was derived from Vero cell line by 20 passages of colonies developed in soft agar, did not definitely change (5.3.7.2pM/mg protein) even after the monolayer became confluency. In contrast, cyclic-AMP level in Vero cell line doubled after the monolayer became confluency. After infection of measles virus, cyclic-AMP in Vero-S cells slightly increased (10.2-13.5 pM/mg protein) until the maximum virus replication and development of the CPE (cytopathic effect), but decreased (7.4.9.7pM/mg protein) thereafter. Multiplication of Vero cells was partially inhibited with 1.10mM and completely with 100mM exogeneous cyclic AMP. But 100mM exogeneous cyclic-AMP did not morphologically affect after monolayer of Vero cells became confluency. Five to 10mM exogeneous cyclic-AMP added at the time of infection decreased the 48 hr-virus yield of measles virus in infected Vero-S cells. In a persistently infected Vero cell line with measles virus, 448-PI-Vero, cells multiplied until 8 days after passage, and infective virus increased sharply until 4 days but slightly thereafter. Cyclic AMP levels in the 448-PI-Vero continued without definite change (8.3.9.5pM/mg protein) through 16 days after passage, but did not decrease as the infected Vero-S cells. Virus yield from 448-PI-Vero decreased with 10mM exogeneous cyclic AMP after 72hr of adding the drug.<BR>From these results, it is apparent that intracellular cyclic-AMP level does not decrease with the production of virus in the persistently infected cells, and inhibition of virus growth with exogeneous cyclic-AMP takes a time lag for the expression; the mechanism which maintains the persistent infection of 448-PI-Vero was related with cyclic-AMP.

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